Reproduction Biotechnology in Buffalo is the first book of its kind to provide readers with comprehensive information to augment bubaline production. This insightful and thought-provoking collection of chapters aims to review cutting-edge technologies and scientific advances in livestock production and conservation. The book begins with discussion on general aspects of global distribution and domestication of water buffalo (Bubalus bubalis) and its role as indispensable and multipurpose livestock species. Embryo transfer, in vitro embryo production, cryopreservation of oocytes, embryos and somatic cells are reviewed. The authors then describe recent developments in sex pre-selection, animal cloning, stem cell technology and biopharming using transgenic animals before concluding chapters on animal embryo-developmental block, nuclear reprogramming and role of molecular biological and genomic applications in overcoming the key problems currently associated with advanced assisted reproduction technologies. Bottlenecks associated with these technologies are identified and possible suggestions to overcome their major limitations are proposed. The chapters include figures, original illustrations, photographs and tables. The target readers for the book include undergraduate and postgraduate students of various agriculture and animal husbandry academia, teachers, professionals and researchers in applied animal sciences including biotechnology, physiology, reproduction and nutrition.