In these rigorous and challenging essays, writers from Aotearoa and Turtle Island (Canada and the United States of America) explore the well-being of takatapui, two-spirit, and Maori and Indigenous LGBTQI+ communities. Themes include resistance, reclamation, empowerment, transformation and healing. Central to Honouring Our Ancestors is the knowledge that, before colonisation, Indigenous peoples had their own healthy understandings of gender, sexual identities and sexuality. Some of these understandings have survived the onslaught of colonisation; others require decolonisation so that our Indigenous nations can begin to heal. Through this lens, the writers gathered here contribute their knowledge and experience of structural and social change. This collection was inspired by two major research projects: the HONOR Project, which investigated well-being in American Indian and Alaskan Native two-spirit communities, and the Honour Project Aotearoa, which investigated Kaupapa Maori strengths-based understandings of the health and well-being of takatapui and Maori LGBTQI+ communities. Edited by Alison Green and Leonie Pihama, Honouring Our Ancestors upholds the independent authorities and languages that distinguish our Indigenous nations and celebrates the relationships that bind us. Decolonised Indigenous knowledges are offered as a wellspring of unlimited potential for Indigenous communities and nations everywhere.