This is a comprehensive research guide that describes both the key new techniques and more established methods. Every chapter discusses the merits and limitations of the various approaches and then provides selected tried- and-tested protocols. It presents the most accessible and comprehensive introduction available to the culture and experimental manipulation of animal cells. The most complete resource on the techniques, equipment, principles, and practices of animal cell culture, this text offers a complete background related to growth of animal cells in culture. It is designed specifically to provide powerful research opportunities for those interested in perusing work in large scale production of stem cells for clinical application.