This book collects and integrates the results of an extensive research program conducted by the authors over the past two decades. It spans the disciplines of transportation science, operations management, and behavioral economics, and consists of 16 chapters previously published in peer-reviewed academic journals and grouped under three topical sections: queueing, route choice, and departure time. The book focuses on strategic interactions in directed networks and laboratory experiments carefully designed to test the descriptive validity of the underlying theoretical models. The research question that unifies the chapters is: do the conclusions of theoretical literature account for the decisions of network users in controlled laboratory experiments? With several major qualifications, this book answers this question affirmatively.