This 2nd revised edition equals the popular 1st edition in providing a clear and detailed overview of cell culture. It presents information on: characteristics of cultured cells; culture vessels; glassware preparation and sterilisation techniques; subculturing; primary cells; cell culture media; techniques; contamination; the cell cycle; cell synchronisation; use of radioactive isotopes in cell culture; cell mutants and cell hybrids; viruses; and differentiation in cell cultures.Reviews on the 1st edition: ''.. the book provides an excellent insight into the way cell culture techniques can be employed in the analytical study of cellular biology.'' - Trends in Biochemical Sciences ''It is well written in a concise, easy-to-read style which stimulates the interest of the reader....'' - Science Tools ''A useful handbook on principles and practice.'' - Immunology Today