This manual is designed to satisfy the needs of students enrolled in؟ B.Sc. degree program in Biological, Microbiological, Agricultural and health professions. It provides؟ well balanced and chosen collection of relevant practical Microbiology Laboratory experiments. Students will perform experiments and report on quantitative as well as descriptive data pertaining to the concept they are tackling. The experiments in this manual stresses the quantitative methods, experimental controls, data analysis as well as report writing. The experiments were designed to provide maximum flexibility although each experiment represents؟ well defined concept, several experiments may be performed concurrently depending upon availability of tools and equipments as well as time constraints and students numbers in each laboratory session. Several appendixes appear at the end of the manual which include staining techniques, media composition and some bacterial diagnostic plates.