Volume 608 of the series Methods in Enzymology covers key aspects of enzyme discovery, engineering tools and platforms, and examples of applications in the enzymology of synthetic biology. - Detailed methods for laboratory use of enzymes in synthetic biology applications - Informative case history examples illustrating how enzyme and metabolic engineering are used to generate new products - Emphasises latest developments in laboratory automation for the engineering of biology - Covers many aspects of the design, build, test, learn cycle used in synthetic biology