《蛋白质纯化与分析技术》以蛋白质纯化与分析技术为核心，对其基本原理及应用实例做了详细的阐述。主要内容包括：蛋白质纯化的依据与设计、蛋白质的提取技术、蛋白质的纯化技术、特征蛋白质的纯化技术以及定量与分析检测技术。其中，蛋白质的提取技术包括蛋白质提取的准备、蛋白质的总提取、不同来源蛋白质的具体提取策略；蛋白质的纯化技术包括沉淀技术、透析和超滤、电泳技术和色谱技术；特征蛋白质的纯化技术包括抗体、钙调蛋白、膜蛋白、生物活性酶（尿激酶、激肽释放酶和植物溶菌酶）和重组蛋白包涵体的纯化；蛋白质的定量、储存与结晶包括提纯过程中的定量、提纯后的纯度标准、纯品蛋白质的储存和蛋白质的结晶等内容；蛋白质的分析与检测包括氨基酸序列分析、蛋白质免疫印迹、酶联免疫分析、质谱技术和肽图谱技术。（This book focuses on protein purification and analysis technology, expounding its basic principles and application examples. It mainly covers the basis and design of protein purification, protein extraction technology, protein purification technology, purification technology of characteristic proteins and the quantitative and analytical detection technology. Among them, the protein extraction technology involves the preparation for protein extraction, the total extraction of protein, the specific extraction strategies for proteins of different sources; the protein purification technology includes the precipitation technology, dialysis and ultrafiltration, electrophoresis and chromatography technology; the purification technology of characteristic proteins covers the purification of antibodies, calmodulin, membranin, bioactive enzymes (urokinase, kallikrein and plant lysozyme) and recombinant protein inclusion bodies; the quantification, storage and crystallization of proteins include the quantification in the purification process, purity standards after purification, storage of pure protein and protein crystallization; and protein analysis and detection involve amino acid sequence analysis, protein immunoblotting, enzyme-linked immunoassay, mass spectrometry and peptide mapping technology.）