This e-book contains a set of papers presented at the "Second International Conference on Whole Life Urban Sustainability and its Assessment", held on 22-24 April 2009 in Loughborough, UK. The aim of the event was to bring together leading international researchers, professionals, tool developers, policy makers and other stakeholders who are involved in the field of urban sustainability assessment to exchange ideas and knowledge on how to meet the assessment needs of urban decision-makers. A selection of papers from this conference are included in this e-book.