In order to increase the healing opportunities of cancer, it is important to impair the dissemination and the spreading of cancer cells from the initial tumor and the formation of metastases in other organs or tissues of the human body. The underlying physical principles of these oncological processes are a major constituent of the research field highlighted in Physics of Cancer. This revised second edition is improved linguistically with multiple increases of the number of figures and the inclusion of several novel chapters such as actin filaments during matrix invasion, microtubuli during migration and matrix invasion, nuclear deformability during migration and matrix invasion, and the active role of the tumor stroma in regulating cell invasion. Part of Biophysical Society series.