This volume of Methods in Enzymology brings together a comprehensive collection of experimental approaches and mechanistic insights into enzymes work on RNAs—molecular catalysts at the heart of RNA metabolism, regulation, and therapeutics. Enzymes work on RNAs are essential for a wide array of biological processes, from gene expression and RNA processing to translation control, RNA silencing, and genome integrity. The volume is organized into six thematic sections that reflect the multifaceted roles of RNA enzymes in biology and medicine. Together, these chapters—authored by leading experts in the field—provide an essential resource for researchers seeking to understand and manipulate RNA enzyme function using state-of-the-art biochemical, biophysical, and therapeutic approaches. - Emerging technologies understanding RNA modifications and structures - RNA modifying and processing enzymes - A combination of chemical, biochemical, and cell biology tools