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《物种起源》主要讲述了生物变异、生存斗争、杂种性质、地质记录的不完整性、生物分布等内容。在本书中，达尔文首次提出了进化论的观点，证明物种的演化是通过自然选择和人工选择的方式实现的，反对唯心神造论和物种不变论，使当时生物学各领域的概念和观念发生了巨大变化。
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