Mononuclear Non-heme Iron Dependent Enzymes Part B
ebook ∣ Methods in Enzymology
By Jennifer Bridwell-Rabb
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Mononuclear Non-heme Iron Dependent Enzymes, Volume 704, Part B focuses on methods for studying, characterizing, and leveraging the chemistry of mononuclear non-heme iron dependent enzymes. Chapters in this new release include Observing extradiol dioxygenases in action through a crystalline lens, Methods used to determine the structure of the oxygenase component of naphthalene 1,2-dioxygenase, Expression, purification, kinetics, and crystallization of non-heme mononuclear iron enzymes: biphenyl, phthalate, terephthalate dioxygenase, Substitution of the Mononuclear, Non-heme Iron Cofactor in Lipoxygenases for Structural Studies: Non-heme iron enzyme dependent reactions, and much more.Other sections cover Cyclopropanation and aziridination catalyzed by non-heme iron and 2-oxogluatarate dependent enzymes, Expression, purification, and characterization of non-heme Iron-dependent mono-oxygenase OzmD in Oxazinomycin Biosynthesis, Isonitrile Biosynthesis by a Non-Heme Iron(II)-Dependent Oxidases/Decarboxylase, Functional analysis of an a-ketoglutarate-dependent non-heme iron oxygenase in fungal meroterpenoid biosynthesis, Spectroscopic and Computational Studies of a Bifunctional Iron- and 2-oxoglutarate Dependent Enzyme, AsqJ, and much more.
- Provides detailed articles regarding how to study the structures and mechanisms of mononuclear non-heme iron dependent enzymes
- Guides readers on how to use partner proteins in non-heme iron enzyme catalysis
- Includes strategies to employ mononuclear non-heme iron enzymes in biocatalytic applications