This Book titled Molecular, Enzymatic and Biochemical Assays for Agricultural Pests focuses on fundamental molecular techniques, encompassing DNA extraction and sequence submission to the NCBI database. Additionally, it delves into protein estimation, immunoblotting, and real-time PCR.Moreover, the book features biochemical assays, such as glucose and protein triglycerides estimation, as well as stress-responsive enzymes. It is expected that researchers from diverse fields will find these techniques valuable for their own investigations.