The book has been written for undergraduate as well as Post Graduate students covering major aspect of practical based literatures of Principle of Biotechnology and Genetics. 1. Completely as per the prescribed syllabus of Biotechnology meant for Indian Universities. 2. Written in a simple and lucid language which is easy to understand. 3. Presented in point-wise format. 4. Contains 22 chapters, dealing the landmark achievements in biotechnology, bioinstrumentations, supporting methodology, tissue culture techniques, genetic engineering techniques, and its applications. 5. The glossary of technical terms, abbreviations, unit detail, and references are mentioned at the end. This book will serve immensely for teachers and students in the field of biotechnology.